Research Paper Volume 13, Issue 13 pp 17499—17515

Downregulation of LINC00665 suppresses the progression of lung adenocarcinoma via regulating miR-181c-5p/ZIC2 axis

LINC00665 downregulation repressed tumor growth via regulating miR-181c-5p/ZIC2. (A, B) Tumor-bearing mice were constructed to assess the tumorigenesis of SK-LU-1 cells with sh-NC+inhibitor-NC, sh-LINC00665+inhibitor-NC or sh-LINC00665+inhibitor-miR-181c-5p stable transfection. (C) RT-PCR technology was applied to test the mRNA levels of LINC00665, miR-181c-5p, ZIC2, HOXA1 and HOX11 in tumors. (D) The protein levels of ZIC2, HOXA1 and HOXA11 in mouse tumor tissues were determined by western blotting assay. (*p0.05, vs. inhibitor-NC+sh-NC group; #p0.05, vs. inhibitor-miR-181c-5p+sh-NC group).

Figure 9. LINC00665 downregulation repressed tumor growth via regulating miR-181c-5p/ZIC2. (A, B) Tumor-bearing mice were constructed to assess the tumorigenesis of SK-LU-1 cells with sh-NC+inhibitor-NC, sh-LINC00665+inhibitor-NC or sh-LINC00665+inhibitor-miR-181c-5p stable transfection. (C) RT-PCR technology was applied to test the mRNA levels of LINC00665, miR-181c-5p, ZIC2, HOXA1 and HOX11 in tumors. (D) The protein levels of ZIC2, HOXA1 and HOXA11 in mouse tumor tissues were determined by western blotting assay. (*p<0.05, vs. inhibitor-NC+sh-NC group; #p<0.05, vs. inhibitor-miR-181c-5p+sh-NC group).