Research Paper Advance Articles

Glioma stem cell-derived exosomal miR-944 reduces glioma growth and angiogenesis by inhibiting AKT/ERK signaling

Overexpression of miR-944 inhibits proliferation and migration of HUVECs. (A) RT-qPCR analysis shows miR-944 expression in blank control, and agomiR-NC, agomiR-944, and antagomiR-944-transfected HUVECs. (B) CCK-8 assay results show viability of blank control, agomiR-NC-, agomiR-944-, and antagomiR-944-transfected HUVECs. (C, D) Immunofluorescence assay results demonstrate staining of blank control, and agomiR-NC-, agomiR-944-, and antagomiR-944-transfected HUVECs with anti-Ki67 antibody to determine the percentage of Ki67-positive HUVECs. Note: Ki67 is a proliferation marker. (E, F) Transwell migration assay results demonstrate the migration ability of blank control, and agomiR-NC-, agomiR-944-, and antagomiR-944-transfected HUVECs. **P

Figure 2. Overexpression of miR-944 inhibits proliferation and migration of HUVECs. (A) RT-qPCR analysis shows miR-944 expression in blank control, and agomiR-NC, agomiR-944, and antagomiR-944-transfected HUVECs. (B) CCK-8 assay results show viability of blank control, agomiR-NC-, agomiR-944-, and antagomiR-944-transfected HUVECs. (C, D) Immunofluorescence assay results demonstrate staining of blank control, and agomiR-NC-, agomiR-944-, and antagomiR-944-transfected HUVECs with anti-Ki67 antibody to determine the percentage of Ki67-positive HUVECs. Note: Ki67 is a proliferation marker. (E, F) Transwell migration assay results demonstrate the migration ability of blank control, and agomiR-NC-, agomiR-944-, and antagomiR-944-transfected HUVECs. **P < 0.01 vs. NC group. HUVECs, human umbilical vein endothelial cells; NC, negative control.