Figure 7. Analysis of CDKN2A or ACTA2 promoter activity. (A) Schematic illustration shows 4 promoter constructs with different lengths and location of Y-box mutation. The indicated CDKN2A promoter constructs were transfected into cells, and the promoter-luciferase activity was measured for the 3 kb construct in BJ vs. BJ5ta cells (B), 3 and 2 kb promoters (C), and 1.2 kb WT vs. MT in both cells (D). (E) The ACTA2 promoter construct was measured similarly as (B). All values shown were after normalizing to the Renilla luciferase activity. The results were expressed as relative light units. N = 5 in (B), 6 in (C and D), and 3 in (E). *P < 0.05. ***P < 0.001. ****P < 0.0001.