Research Paper Volume 13, Issue 13 pp 17568—17591

Aging-dependent loss of GAP junction proteins Cx46 and Cx50 in the fiber cells of human and mouse lenses accounts for the diminished coupling conductance

Differential expression patterns of the connexins Cx43, Cx46 and Cx50 in the epithelial cells of the αA-/- and αB-/- mouse lenses (Figure 10A–10C), and in the fiber cells of the αA-/- mouse lenses (Figure 10D–10E). (A) Western blot analysis of Cx43 and Cx50 in the lens epithelial cells of the αA-/- and αB-/- mice. β-Tubulin was showed as a loading control. (B) & (C) Quantification of the western blot results in (A). (D) Western blot analysis of Cx43 and Cx50 in the lens fiber cells of the αA-/- mice. α-Actinin was showed as a loading control. (E) Quantification of the western blot results in (D). NS, not significant, *p **p ***p

Figure 10. Differential expression patterns of the connexins Cx43, Cx46 and Cx50 in the epithelial cells of the αA-/- and αB-/- mouse lenses (Figure 10A10C), and in the fiber cells of the αA-/- mouse lenses (Figure 10D10E). (A) Western blot analysis of Cx43 and Cx50 in the lens epithelial cells of the αA-/- and αB-/- mice. β-Tubulin was showed as a loading control. (B) & (C) Quantification of the western blot results in (A). (D) Western blot analysis of Cx43 and Cx50 in the lens fiber cells of the αA-/- mice. α-Actinin was showed as a loading control. (E) Quantification of the western blot results in (D). NS, not significant, *p < 0.05, **p < 0.01, ***p < 0.001.