Research Paper Volume 13, Issue 15 pp 19260—19271

Downregulation of lncRNA SBF2-AS1 inhibits hepatocellular carcinoma proliferation and migration by regulating the miR-361-5p/TGF-β1 signaling pathway

Relationship between miR-361-5p and TGF-β1. (A) Prediction of the binding site of miR-361-5p and TGF-β1. (B) Luciferase reporter assay verified the direct binding effect between miR-361-5p and TGF-β1. (C) The effect of AMO-361-5p on miR-361-5p in HepG2 cells. (D) The influence of AMO-361-5p on miR-361-5p in Hep3B cells. (E) The influence of miR-361-5p knockdown on TGF-β1 mRNA levels in HepG2 cells. (F) The influence of miR-361-5p knockdown downregulation on TGF-β1 mRNA levels in Hep3B cells. (G) The influence of miR-361-5p knockdown on TGF-β1 protein levels in HepG2 cells. (H) The influence of miR-361-5p knockdown downregulation on TGF-β1 protein expression in Hep3B cells. The obtained data were expressed as mean ± SEM. * P

Figure 3. Relationship between miR-361-5p and TGF-β1. (A) Prediction of the binding site of miR-361-5p and TGF-β1. (B) Luciferase reporter assay verified the direct binding effect between miR-361-5p and TGF-β1. (C) The effect of AMO-361-5p on miR-361-5p in HepG2 cells. (D) The influence of AMO-361-5p on miR-361-5p in Hep3B cells. (E) The influence of miR-361-5p knockdown on TGF-β1 mRNA levels in HepG2 cells. (F) The influence of miR-361-5p knockdown downregulation on TGF-β1 mRNA levels in Hep3B cells. (G) The influence of miR-361-5p knockdown on TGF-β1 protein levels in HepG2 cells. (H) The influence of miR-361-5p knockdown downregulation on TGF-β1 protein expression in Hep3B cells. The obtained data were expressed as mean ± SEM. * P < 0.05, ** P < 0.01 vs. si-NC; n=3.