Research Paper Volume 13, Issue 17 pp 21090—21101

Effect of the Notch1-mediated PI3K-Akt-mTOR pathway in human osteosarcoma

Notch1 induced by doxycycline has blocked PI3K/Akt/mTOR signaling pathway in human osteosarcoma cells. Levels of PI3K, p-PI3K, Akt, p-Akt, mTOR, p-mTOR were analyzed by western blotting. *P

Figure 6. Notch1 induced by doxycycline has blocked PI3K/Akt/mTOR signaling pathway in human osteosarcoma cells. Levels of PI3K, p-PI3K, Akt, p-Akt, mTOR, p-mTOR were analyzed by western blotting. *P<0.05, significantly different compared with the untreated control group. All the experiments were conducted in triplicates.