Figure 4. WNT2 was a directed target of miR-335-3p. (A) The binding bases of miR-335-3p and WNT2 from Targetscan. (B) Wild type and mutant WNT2 was transfected into HEK293 cells with or without miR-335-3p, and luciferase assay was to evaluate the binding. BT549 cells were transfected with miR-335-3p or AMO-miR-335-3p, (C) the mRNA level and (D) the protein level of WNT2 was detected. (E–F) The expression of WNT2 in TNBC tissues and cells was determined by qRT-PCR. Data are mean ± SD; *P < 0.05, Abbreviation: ns: no statistical significance.