Research Paper Volume 13, Issue 14 pp 18340—18359

CircSPIDR acts as a tumour suppressor in cervical adenocarcinoma by sponging miR-431-5p and regulating SORCS1 and CUBN expression

CircSPIDR overexpression inhibits HeLa cell growth in vivo. (A) HeLa cells expressing circSPIDR or the vector control were inoculated into BALB/c nude mice (n = 6/group) to establish subcutaneous xenograft tumors. Representative images of nude mice bearing CADC tumors are shown. (B) Growth curves and representative images of isolated xenograft tumours. The volume of the local tumors was measured. (C) Hematoxylin and eosin staining was performed and Ki-67 protein expression was evaluated in the xenograft tumors. (D, E) Representative images of nude mice intraperitoneally transplanted with HeLa/circSPIDR and HeLa/vector cells. ***P

Figure 3. CircSPIDR overexpression inhibits HeLa cell growth in vivo. (A) HeLa cells expressing circSPIDR or the vector control were inoculated into BALB/c nude mice (n = 6/group) to establish subcutaneous xenograft tumors. Representative images of nude mice bearing CADC tumors are shown. (B) Growth curves and representative images of isolated xenograft tumours. The volume of the local tumors was measured. (C) Hematoxylin and eosin staining was performed and Ki-67 protein expression was evaluated in the xenograft tumors. (D, E) Representative images of nude mice intraperitoneally transplanted with HeLa/circSPIDR and HeLa/vector cells. ***P < 0.001.