Figure 6. SORCS1 and CUBN are the target genes of miR-431-5p and circSPIDR. (A) miR-431-5p-target gene regulatory network. (B) qRT-PCR analysis of candidate target gene expression in CADC cells transfected with miR-431-5p inhibitors. (C, D) qRT-PCR analysis of candidate target gene expression in CADC cells transfected with the circSPIDR expression vector (C) or siRNAs against circSPIDR (D). (E) Luciferase activity of the 3′UTRs of SORCS1 and the CUBN in 293T cells co-transfected with miR-431-5p mimics. NS, not significant; *P < 0.05; **P < 0.01.