Research Paper Volume 13, Issue 13 pp 16922—16937

A model of the aged lung epithelium in idiopathic pulmonary fibrosis

7-day bleomycin protocol activates the mitochondria unfolded protein response and induces expression of mitochondrial chaperones in alveolar epithelial cells. (A) WB for ATF4 in controls vs. chronically injured lung epithelial cells (with β-actin loading control). Densitometry is shown on the right. (B) Transcript levels for Hsp60, Hsp10 and Hps90 in controls vs. chronically injured MLE12 cells. (C) WB for Clpp and Lonp1 in control and bleomycin injured cells (with β-actin loading control). Densitometry is shown on the right. (D, E) WB for SIRT3, FOXO3a and SOD2 in control and bleomycin injured MLE12 cells (with β-actin loading control). Densitometry is shown on the right. Statistical significance was assessed by unpaired Student’s t-test for two groups. * p

Figure 3. 7-day bleomycin protocol activates the mitochondria unfolded protein response and induces expression of mitochondrial chaperones in alveolar epithelial cells. (A) WB for ATF4 in controls vs. chronically injured lung epithelial cells (with β-actin loading control). Densitometry is shown on the right. (B) Transcript levels for Hsp60, Hsp10 and Hps90 in controls vs. chronically injured MLE12 cells. (C) WB for Clpp and Lonp1 in control and bleomycin injured cells (with β-actin loading control). Densitometry is shown on the right. (D, E) WB for SIRT3, FOXO3a and SOD2 in control and bleomycin injured MLE12 cells (with β-actin loading control). Densitometry is shown on the right. Statistical significance was assessed by unpaired Student’s t-test for two groups. * p< 0.05, **P < 0.01, ***P < 0.001 versus control group, n=6.