Figure 4. 7-day bleomycin protocol alters cellular metabolism in alveolar epithelial cells. (A) Levels of glycolytic metabolites in control and bleomycin injured cells. (B) Extracellular acidification rate in control and bleomycin injured cells. (C–E) Tricarboxylic acid (TCA) cycle intermediates, amino acid levels and urea cycle intermediates in control and bleomycin injured cells. Statistical comparisons were performed using two-way analysis of variance (ANOVA) followed by Sidak post hoc analysis in order to adjust for multiple comparisons. * p< 0.05, **P < 0.01, ***P < 0.001 versus control group, n=6.