Figure 2. ZC3HAV1 improves pancreatic cancer cell proliferation and G1/S phase transition. (A–D) The transfection efficiency of ZC3HAV1 was measured by qRT-PCR and western blot in pancreatic cancer cells. (E, F) The effect of ZC3HAV1 on the proliferation of pancreatic cancer cells was found by CCK-8 assay. (G, H) Flow cytometry dissection of the cell cycle in pancreatic cancer cells after transfection was done. (K–N) Carried out cell colony formation assay in PANC-1 and MIA PaCa-2 cells after transfection. (I, J) Western blotting shows cell cycle protein Cyclin D1 and CDK2 expression in PANC-1 and MIA PaCa-2 cells after transfection. Data appeared to be average ± SD, *P< 0.05, **P< 0.01.