Figure 12. HCT116 ATG16L1 A300 cells make less GRP78 and have a weaker stimulation of eIF2α phosphorylation by AR12 or neratinib. HCT116 (ATG16L1 T300 or ATG16L1 A300) cells were treated with vehicle control, AR12 (2 mM) or neratinib (50 nM). Cells were fixed in place after 6h and the expression of the indicated proteins and phospho-proteins determined by in-cell immuno-staining. (n = 3 +/-SD). * p < 0.05 less than vehicle control; # p < 0.05 greater than vehicle control; ¶ p < 0.05 less than corresponding basal value in ATG16L1 T300 cells; § p < 0.05 less than corresponding stimulated value in ATG16L1 cells; ▼ p < 0.05 less than corresponding value in AR12 treated cells.