Research Paper Advance Articles

Exosomal miRNA-205 promotes breast cancer chemoresistance and tumorigenesis through E2F1

M/T-Exo miRNA-205 affects breast cancer cells (BCCs) proliferation, migration, and invasion via targeting E2F1. (A–C) The abilities of colony formation, migration, and invasion in M/T-Exo-cocultured BCCs treated with DMSO or GW4869. (D) The protein expression of Akt and the phosphorylation of Akt at Ser 473 (p-Akt Ser 473) in M/T-Exo-cocultured BCCs treated with DMSO or GW4869. (E–G) The abilities of colony formation, migration, and invasion in M/T-Exo-cocultured BCCs treated with the negative control miRNA-205 inhibitor (NCi), miRNA-205 inhibitor (205i), or the combination of 205i and E2F1 siRNA (siE2F1). (H) The protein expression of Akt and the phosphorylation of Akt at Ser 473 (p-Akt Ser 473) in M/T-Exo-cocultured BCCs treated with the negative control miRNA-205 inhibitor (NCi), miRNA-205 inhibitor (205i), or the combination of 205i and E2F1 siRNA (siE2F1). Values are means ± SD. *P **P ***P

Figure 6. M/T-Exo miRNA-205 affects breast cancer cells (BCCs) proliferation, migration, and invasion via targeting E2F1. (AC) The abilities of colony formation, migration, and invasion in M/T-Exo-cocultured BCCs treated with DMSO or GW4869. (D) The protein expression of Akt and the phosphorylation of Akt at Ser 473 (p-Akt Ser 473) in M/T-Exo-cocultured BCCs treated with DMSO or GW4869. (EG) The abilities of colony formation, migration, and invasion in M/T-Exo-cocultured BCCs treated with the negative control miRNA-205 inhibitor (NCi), miRNA-205 inhibitor (205i), or the combination of 205i and E2F1 siRNA (siE2F1). (H) The protein expression of Akt and the phosphorylation of Akt at Ser 473 (p-Akt Ser 473) in M/T-Exo-cocultured BCCs treated with the negative control miRNA-205 inhibitor (NCi), miRNA-205 inhibitor (205i), or the combination of 205i and E2F1 siRNA (siE2F1). Values are means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. At least three replicates were available for analysis in each treatment group.