Research Paper Volume 13, Issue 14 pp 18527—18544

LncRNA CCAT1 promotes prostate cancer cells proliferation, migration, and invasion through regulation of miR-490-3p/FRAT1 axis

miR-490-3p inhibited cell proliferation, migration, and invasion in PCa cells. (A) Transfection efficiency of miR-490-3p mimics and inhibitor in LnCaP and PC3 cells. Compared to the NC group, PCa cells treated with miR-mimics had higher miR-490-3p expression, while those treated with miR-inhibitor had lower miR-490-3p expression. (B) Cell viability of LnCaP and PC3 cells with different treatments. Cell viability was suppressed in miR-mimics group and promoted in the miR-inhibitor group. (C) EdU staining of LnCaP and PC3 cells with different treatments. Overexpression of miR-490-3p suppressed cell proliferation, while the down-regulation of miR-490-3p promoted cell proliferation in PCa cells. Scale bar: 50 μm. (D) Cell apoptosis detection of LnCaP and PC3 cells with different treatments. Overexpression of miR-490-3p induced cell apoptosis in PCa cells, while the down-regulation of miR-490-3p suppressed the cell apoptosis. (E, F) Cell migration and invasion of LnCaP and PC3 cells in Transwell assays. miR-490-3p mimics treatment suppressed cell migration and invasion while the miR-490-3p inhibitor treatment activated these cell behaviors in PCa cells. (G) miR-490-3p overexpression up-regulated the protein expression of E-cadherin yet decreased the protein expression of N-cadherin and Vimentin, while miR-490-3p inhibition showed the opposite results. *P P P P

Figure 5. miR-490-3p inhibited cell proliferation, migration, and invasion in PCa cells. (A) Transfection efficiency of miR-490-3p mimics and inhibitor in LnCaP and PC3 cells. Compared to the NC group, PCa cells treated with miR-mimics had higher miR-490-3p expression, while those treated with miR-inhibitor had lower miR-490-3p expression. (B) Cell viability of LnCaP and PC3 cells with different treatments. Cell viability was suppressed in miR-mimics group and promoted in the miR-inhibitor group. (C) EdU staining of LnCaP and PC3 cells with different treatments. Overexpression of miR-490-3p suppressed cell proliferation, while the down-regulation of miR-490-3p promoted cell proliferation in PCa cells. Scale bar: 50 μm. (D) Cell apoptosis detection of LnCaP and PC3 cells with different treatments. Overexpression of miR-490-3p induced cell apoptosis in PCa cells, while the down-regulation of miR-490-3p suppressed the cell apoptosis. (E, F) Cell migration and invasion of LnCaP and PC3 cells in Transwell assays. miR-490-3p mimics treatment suppressed cell migration and invasion while the miR-490-3p inhibitor treatment activated these cell behaviors in PCa cells. (G) miR-490-3p overexpression up-regulated the protein expression of E-cadherin yet decreased the protein expression of N-cadherin and Vimentin, while miR-490-3p inhibition showed the opposite results. *P < 0.05, **P < 0.01, compared with the NC mimics group; #P < 0.05, ##P < 0.01, compared with the NC inhibitor group.