Figure 9. FRAT1 overexpression reversed the effects of CCAT1 suppression in PCa cells. (A) mRNA expression of FRAT1 in LnCaP and PC3 cells transfected with si-CCAT1 or si-CCAT1+pcDNA-FRAT1. (B) The results of MTT assays indicated that down-regulation of CCAT1 suppressed cell viability, and the suppressed ability was counteracted by pcDNA-FRAT1. (C) EdU staining results of LnCaP cells and PC3 cells treated with si-CCAT1 or si-CCAT1+pcDNA-FRAT1. Scale bar: 50 μm. (D) Flow cytometry detection of the cell apoptosis changes in LnCaP cells and PC3 cells treated with si-CCAT1 or si-CCAT1+pcDNA-FRAT1. (E, F) Cell migration and invasion were reduced with si-CCAT1 transfection, and recovered by overexpressing FRAT1. (G) Protein expression of FRAT1, E-cadherin, N-cadherin, and Vimentin in PCa cells were detected by western blot. *P < 0.05, **P < 0.01, compared with the si-NC group; #P < 0.05, ##P < 0.01, compared with the si-CCAT1 group.