Figure 3. Expression of circSP3 in HCC tissues and cell lines. (A) CircSP3 levels in 48 pairs of HCC tissues and adjacent normal liver tissues were evaluated using qRT-PCR. (B) The relative circSP3 levels in four HCC cell lines (Hep-3B, Huh-7, Bel-7402 and SMMC-7721) and an immortalized liver cell line (HL-77O2) were determined using qRT-PCR. (C) CircSP3 levels in Hep-3B cells infected with NC or circSP3 plasmids. (D) qRT-PCR was conducted to confirm the knockdown efficiency of si-circSP3-1 and si-circSP3-2. (E) qRT-PCR was conducted to confirm the knockdown efficiency of LV3-circSP3. *p<0.05, **p<0.01 and ***p<0.001.