Research Paper Volume 13, Issue 14 pp 18586—18605

Circular RNA circSP3 promotes hepatocellular carcinoma growth by sponging microRNA-198 and upregulating cyclin-dependent kinase 4

The relationship between circSP3 and miR-198 in HCC. (A) The relative miR-198 levels in four HCC cell lines (Hep-3B, Huh-7, Bel-7402 and SMMC-7721) and a normal liver cell line (HL-77O2) were assessed using qRT-PCR. (B) Correlation analysis between circSP3 and miR-198 levels in HCC tissues. (C) The relative miR-198 levels in Hep-3B cells infected with NC or circSP3 plasmids were detected using qRT-PCR. (D) The relative miR-198 levels in Huh-7 cells infected with si-NC or si-circSP3 plasmids were detected using qRT-PCR. (E) qRT-PCR revealed that ectopic expression of miR-198 reduced circSP3 expression in Huh-7 cells. (F) qRT-PCR demonstrated that knocking down miR-198 increased circSP3 expression in Hep-3B cells. (G) Schematic of predicted wild-type and mutated miR-198 binding sequences in circSP3. (H, I) Luciferase activity in HCC cells co-transfected with circSP3-wt or circSP3-mut and miR-198 mimics or miR-198 mimic controls. (J) FISH revealed that miR-198 colocalized with circSP3 in HCC cells. **p

Figure 6. The relationship between circSP3 and miR-198 in HCC. (A) The relative miR-198 levels in four HCC cell lines (Hep-3B, Huh-7, Bel-7402 and SMMC-7721) and a normal liver cell line (HL-77O2) were assessed using qRT-PCR. (B) Correlation analysis between circSP3 and miR-198 levels in HCC tissues. (C) The relative miR-198 levels in Hep-3B cells infected with NC or circSP3 plasmids were detected using qRT-PCR. (D) The relative miR-198 levels in Huh-7 cells infected with si-NC or si-circSP3 plasmids were detected using qRT-PCR. (E) qRT-PCR revealed that ectopic expression of miR-198 reduced circSP3 expression in Huh-7 cells. (F) qRT-PCR demonstrated that knocking down miR-198 increased circSP3 expression in Hep-3B cells. (G) Schematic of predicted wild-type and mutated miR-198 binding sequences in circSP3. (H, I) Luciferase activity in HCC cells co-transfected with circSP3-wt or circSP3-mut and miR-198 mimics or miR-198 mimic controls. (J) FISH revealed that miR-198 colocalized with circSP3 in HCC cells. **p<0.01, ***p<0.001.