Research Paper Volume 13, Issue 14 pp 18586—18605

Circular RNA circSP3 promotes hepatocellular carcinoma growth by sponging microRNA-198 and upregulating cyclin-dependent kinase 4

CircSP3 promotes HCC cell proliferation, migration and invasion by inducing CDK4. CircSP3-overexpressing Hep-3B cells were infected with CDK4-homo-520 (CDK4 knockdown) or CDK4-homo-520 control plasmids, and circSP3-knockdown Huh-7 cells were infected with CDK4-overexpressing or CDK4 control plasmids. (A, B) CCK-8, (C, D) Transwell migration and (E, F) Transwell invasion assays were conducted to assess cell proliferation, migration and invasion, respectively. (G, H) The effects of circSP3 and CDK4 expression plasmids on CDK4 expression were quantified using Western blotting. *p

Figure 8. CircSP3 promotes HCC cell proliferation, migration and invasion by inducing CDK4. CircSP3-overexpressing Hep-3B cells were infected with CDK4-homo-520 (CDK4 knockdown) or CDK4-homo-520 control plasmids, and circSP3-knockdown Huh-7 cells were infected with CDK4-overexpressing or CDK4 control plasmids. (A, B) CCK-8, (C, D) Transwell migration and (E, F) Transwell invasion assays were conducted to assess cell proliferation, migration and invasion, respectively. (G, H) The effects of circSP3 and CDK4 expression plasmids on CDK4 expression were quantified using Western blotting. *p<0.05, **p<0.01 and ***p<0.001.