Figure 5. THBS4 mediates BM-MSCs to promote the migration and tube formation of HUVECs in vitro. (A) Transfection of recombinant lentiviral vectors into BM-MSCs. (B–E) qRT-PCR and Western blot analysis of THBS4 expression in BM-MSCs of both experiments transfected as indicated. n= 3 wells per group. (F, H) Transwell assays and quantification of migrated cells showed that THBS4 knockdown in BM-MSCs inhibited their ability to promote HUVEC migration. n= 3 wells in each group. (G, I) Knockdown of THBS4 in BM-MSCs alleviated its ability to promote HUVEC tube formation. n= 5 wells per group.