Figure 5. AEC senescence was inhibited upon FUT8 knockdown in vitro and vivo. (A) P21 and P16 levels were assessed using western blotting analyses in different groups. (B) Representative immunofluorescence images of dual staining for SPC (green) and P21 (red), SPC (green) and P16 (red) are shown in vivo (original magnification, 200×). Data are shown as the mean ± SEM, n ≥ 3 per group. #P < 0.01, ##P < 0.01, *P < 0.01, **P < 0.01. #Indicates the comparison of the control group with the IGF1 group; ##indicates the comparison of the FUT8 siRNA+ IGF1 group with the IGF1 group. *indicates the comparison of the control group with the BLM group; **indicates the comparison of the FUT8shRNA+ BLM group with the BLM group. One-way ANOVA followed by Dunnett’s Multiple Comparison Test.