Research Paper Volume 13, Issue 14 pp 18924—18945
MicroRNA-1252-5p, regulated by Myb, inhibits invasion and epithelial-mesenchymal transition of pancreatic cancer cells by targeting NEDD9
Figure 2. MiR-1252-5p inhibited the proliferation, migration and invasion of PAC cells. (A–D) MTT and CellTiter-Glo luminescent cell viability assays were conducted to detect cell proliferation after transfection with corresponding miRNA vectors in PAC cells. (E) Western blot assays were conducted to examine markers of cell proliferation and apoptosis (PCNA, Bcl-2 and Bax) after transfection with corresponding miRNA vectors in PAC cells. (F–I) Wound healing and Transwell (without or with Matrigel) assays analysis of cell migratory and invasive ability after transfection with corresponding miRNA vectors in PAC cells. **P < 0.01; ***, P < 0.001.