Figure 3. MiR-1252-5p inhibited the EMT process of PAC cells. (A, B) Representative immunohistochemical staining and histograms for E-cad (A) and Vim (B) expression were showed and compared between high- and low-miR-1252-5p expressing PAC tissues. Scale bar, 200μm for upper row and 40μm for lower row. (C) Western blot analysis of EMT markers (E-cad, N-cad, Vim, ZEB1, Snail and Twist) after transfection with corresponding miRNA vectors in PAC cells. GAPDH served as a loading control. E-cad, E-cadherin; N-cad, N-cadherin; Vim, Vimentin. **P < 0.01; ***, P < 0.001.