Research Paper Volume 13, Issue 14 pp 18924—18945

MicroRNA-1252-5p, regulated by Myb, inhibits invasion and epithelial-mesenchymal transition of pancreatic cancer cells by targeting NEDD9

Modulation of NEDD9 partially restored miR-1252-5p-mediated cellular functions in PAC. (A–D) qRT-PCR and western blot analysis of NEDD9 mRNA and protein expression after transfection with corresponding vectors in PAC cells. (E, F) MTT assays were conducted to detect cell proliferation after transfection with corresponding vectors in PAC cells. (G–J) Wound healing and Transwell (without or with Matrigel) assays analysis of cell migratory and invasive ability after transfection with corresponding miRNA vectors in PAC cells. (K, L) Western blotting assays were conducted to detect NEDD9 and EMT markers (E-cad, N-cad, Vim) after transfection with corresponding vectors in PAC cells. (M) IHC assay analysis of the association between miR-1252-5p expression and NEDD9 expression in human PAC tissues. Scale bar, 100μm for upper row and 40μm for lower row. NEDD9, neural precursor cell expressed, developmentally downregulated 9; E-cad, E-cadherin; N-cad, N-cadherin; Vim, Vimentin. *P P P

Figure 5. Modulation of NEDD9 partially restored miR-1252-5p-mediated cellular functions in PAC. (AD) qRT-PCR and western blot analysis of NEDD9 mRNA and protein expression after transfection with corresponding vectors in PAC cells. (E, F) MTT assays were conducted to detect cell proliferation after transfection with corresponding vectors in PAC cells. (GJ) Wound healing and Transwell (without or with Matrigel) assays analysis of cell migratory and invasive ability after transfection with corresponding miRNA vectors in PAC cells. (K, L) Western blotting assays were conducted to detect NEDD9 and EMT markers (E-cad, N-cad, Vim) after transfection with corresponding vectors in PAC cells. (M) IHC assay analysis of the association between miR-1252-5p expression and NEDD9 expression in human PAC tissues. Scale bar, 100μm for upper row and 40μm for lower row. NEDD9, neural precursor cell expressed, developmentally downregulated 9; E-cad, E-cadherin; N-cad, N-cadherin; Vim, Vimentin. *P < 0.05; **P < 0.01; ***, P < 0.001.