Research Paper Volume 13, Issue 14 pp 18978—18992

CircRNA circFOXK2 facilitates oncogenesis in breast cancer via IGF2BP3/miR-370 axis

CircFOXK2-miR-370 interaction is essential for BC metastasis. (A) Putative binding site of miR-370 in the circFOXK2 sequence. (B) Luciferase activity in BT-549 cells treated as indicated. (C) circRIP assay. (D) Expression of miR-370 in circFOXK2-overexpressing/knockdown BT-549 cells. (E) Pearson correlation between the expressions of circFOXK2 and miR-370. (F) Expression of miR-370 in BT-549 cells treated with miR-370 mimic. (G) Expression of miR-370 in BT-549 cells treated with miR-370 inhibitor. (H and J) Rescue experiments for the migration ability of BT-549 cells treated as indicated. Scale bar: 20 μm. (I and K) Rescue experiments for the invasion ability of BT-549 cells treated as indicated. Scale bar: 20 μm. Data were represented as mean ± SD. Each experimental group had at least three replicates. *p **p ***p

Figure 5. CircFOXK2-miR-370 interaction is essential for BC metastasis. (A) Putative binding site of miR-370 in the circFOXK2 sequence. (B) Luciferase activity in BT-549 cells treated as indicated. (C) circRIP assay. (D) Expression of miR-370 in circFOXK2-overexpressing/knockdown BT-549 cells. (E) Pearson correlation between the expressions of circFOXK2 and miR-370. (F) Expression of miR-370 in BT-549 cells treated with miR-370 mimic. (G) Expression of miR-370 in BT-549 cells treated with miR-370 inhibitor. (H and J) Rescue experiments for the migration ability of BT-549 cells treated as indicated. Scale bar: 20 μm. (I and K) Rescue experiments for the invasion ability of BT-549 cells treated as indicated. Scale bar: 20 μm. Data were represented as mean ± SD. Each experimental group had at least three replicates. *p < 0.05, **p < 0.01, ***p < 0.001.