Figure 7. Treatment with C16+Ang-l can alleviate 3-NP-induced TH and CHAT expression decline and synaptophysin loss. Immunofluorescence images showing (A–I) TH-positive cells (red, dopaminergic neurons; white box in (A–C), nigra substance at low magnification ×40; (D–F) showed the same area at medium magnification ×100; and (G–I) at high magnification ×200), (K–M) CHAT-positive cells (green, cholinergic neurons; blue, Hoechst33342-stained cell nuclei), and (O–Q) the pixel of Syn-positive cells (synapse-associated proteins that showed synaptic plasticity and correlated with cognitive decline) in the control, 3-NP, and 3-NP+C16+Ang-1 groups. Scale bar = 100 μm. (J, N, R) semi-quantitative profiles of TH (J), CHAT (N) and Syn (R) in the control, 3-NP, and 3-NP+C16+Ang-1 groups. aP < 0.05 versus control; bP < 0.05 versus 3-NP-treated mice.