Research Paper Volume 13, Issue 14 pp 19064—19076

Type I collagen promotes tumor progression of integrin β1 positive gastric cancer through a BCL9L/β-catenin signaling pathway

Collagen mediated gastric cancer progression through integrin β1. (A) The proliferation of unsorted or ITGB1-/+ SGC-7901/BGC-823 cells in 72 hours. (B) The 3D colony formation capability of unsorted or ITGB1-/+ SGC-7901/BGC-823 cells in 3D collagen gel. (C) The colony sizes of unsorted or ITGB1-/+ SGC-7901/BGC-823 cells in 3D collagen gel. The scale bar is 30 μm. (D) The ITGB1-/+ SGC-7901 cells were sorted and cultured in 3D collagen gel (6 days) or not. Then the tumorigenic capability of SGC-7901 in NOD-SCID mice were examined. (E) The ITGB1-/+ SGC-7901 cells were sorted and cultured 3D collagen gel (6 days) or not. Then tumor cells were treated with 5-FU (5 μg/ml) and the cell apoptosis was examined. *Indicates P

Figure 2. Collagen mediated gastric cancer progression through integrin β1. (A) The proliferation of unsorted or ITGB1-/+ SGC-7901/BGC-823 cells in 72 hours. (B) The 3D colony formation capability of unsorted or ITGB1-/+ SGC-7901/BGC-823 cells in 3D collagen gel. (C) The colony sizes of unsorted or ITGB1-/+ SGC-7901/BGC-823 cells in 3D collagen gel. The scale bar is 30 μm. (D) The ITGB1-/+ SGC-7901 cells were sorted and cultured in 3D collagen gel (6 days) or not. Then the tumorigenic capability of SGC-7901 in NOD-SCID mice were examined. (E) The ITGB1-/+ SGC-7901 cells were sorted and cultured 3D collagen gel (6 days) or not. Then tumor cells were treated with 5-FU (5 μg/ml) and the cell apoptosis was examined. *Indicates P <0.05, **Indicates P <0.01, n.s indicates no significant difference.