Research Paper Volume 13, Issue 15 pp 19442—19459

CC16-TNF-α negative feedback loop formed between Clara cells and normal airway epithelial cells protects against diesel exhaust particles exposure-induced inflammation

Characterization of sorted cells. (A, B) CC16 mRNA and protein expression in the sorted CC16+ and CC16- cell fraction are detected by RT-PCR and western blot assay. (C, D) Immunofluorescence (IF) staining shows that 98% of the cells in CC16+ sorted fraction express CC16, and all the cells in CC16+ sorted fraction show positive expression of pan-keratin, the epithelial marker (× 100). ***P - fraction.

Figure 1. Characterization of sorted cells. (A, B) CC16 mRNA and protein expression in the sorted CC16+ and CC16- cell fraction are detected by RT-PCR and western blot assay. (C, D) Immunofluorescence (IF) staining shows that 98% of the cells in CC16+ sorted fraction express CC16, and all the cells in CC16+ sorted fraction show positive expression of pan-keratin, the epithelial marker (× 100). ***P < 0.001 vs. CC16- fraction.