Figure 1. Characterization of sorted cells. (A, B) CC16 mRNA and protein expression in the sorted CC16+ and CC16- cell fraction are detected by RT-PCR and western blot assay. (C, D) Immunofluorescence (IF) staining shows that 98% of the cells in CC16+ sorted fraction express CC16, and all the cells in CC16+ sorted fraction show positive expression of pan-keratin, the epithelial marker (× 100). ***P < 0.001 vs. CC16- fraction.