Research Paper Volume 13, Issue 15 pp 19442—19459

CC16-TNF-α negative feedback loop formed between Clara cells and normal airway epithelial cells protects against diesel exhaust particles exposure-induced inflammation

rCC16 relieves DEP-induced bronchial epithelial cell inflammation via activation of autophagy. BEAS-2B cells are incubated with 5 ppm SRM 1650b, or 300 ng/ml rCC16 + 5 ppm SRM 1650b for 12 h. Then the cells and culture supernatants are harvested. (A) LC3B and p62 protein expression in BEAS-2B cells are detected by western blot. (B) Autophagy induction is evaluated by LC3B immunostaining (× 400). Autophagosomes are identified as bright green dots. (C–E) TNF-α, IL-6, and IL-8 level in culture supernatants are detected by ELISA. ***P

Figure 7. rCC16 relieves DEP-induced bronchial epithelial cell inflammation via activation of autophagy. BEAS-2B cells are incubated with 5 ppm SRM 1650b, or 300 ng/ml rCC16 + 5 ppm SRM 1650b for 12 h. Then the cells and culture supernatants are harvested. (A) LC3B and p62 protein expression in BEAS-2B cells are detected by western blot. (B) Autophagy induction is evaluated by LC3B immunostaining (× 400). Autophagosomes are identified as bright green dots. (CE) TNF-α, IL-6, and IL-8 level in culture supernatants are detected by ELISA. ***P < 0.001 vs. ctrl; and #P < 0.05 and ###P < 0.001 vs. DEP group.