Figure 4. Influence of salt RNAi, HSI, and LTE on heart. (A) Heart diastolic period. (B) Heart systolic period. (C) Heart period. (D) Diastolic diameter. (E) Systolic diameter. (F) Fractional shortening. (G) Arrhythmia index. (H) Microscopic image of cardiac function from M-mode trace in 5-week-old Drosophila. It can be observed that heart salt gene overexpression could increase heart period and arrhythmia, and it could reduce fractional shortening. LTE could reduce heart period and arrhythmia, and it could increase fractional shortening in heart salt gene overexpression flies. (I) The heart salt expression level. (J) The heart dTOR expression. (K) Cardiac PGC-1α expression. (L) Cardiac dFOXO expression. (M) Cardiac SOD activity level. (N) Cardiac MDA level. Using a one-way analysis of variance (ANOVA) followed by an LSD test among these groups. Data are represented as means ± SEM. *P<0.05; **P <0.01. Sample size was 30 hearts for each group. For RT-PCR and ELISA, sample size was 80 hearts for each group, and measurements were taken 3 times.