Research Paper Volume 13, Issue 15 pp 19542—19560

Physical exercise prevents age-related heart dysfunction induced by high-salt intake and heart salt-specific overexpression in Drosophila

Influence of heart salt RNAi, HSI, and LTE on the climbing ability and lifespan in Drosophila. (A) The climbing index in 1-week old, 3-week old, and 5-week old flies. (B) Fly population survival (%) curve. The leftmost curve represents the Salt-control+HSI group, of which flies had the shortest lifespan. (C) Influence of heart salt RNAi and HSI on climbing endurance in 1-week old flies. (D) Influence of heart salt RNAi and HSI on climbing endurance in 3-week old flies. (E) Influence of heart salt RNAi and HSI on climbing endurance in 5-week old flies. (F) Influence of heart salt RNAi, exercise, and HSI on climbing endurance in 1-week old flies. (G) Influence of heart salt RNAi, exercise, and HSI on climbing endurance in 3-week old flies. (H) Influence of heart salt RNAi, exercise, and HSI on climbing endurance in 5-week old flies. For climbing ability measurement, the sample size was about 100 flies for each group. For climbing index, using a one-way analysis of variance (ANOVA) followed by an LSD test among different groups. For lifespan, the sample size was 200-220 flies for each group. P-values for lifespan curves and climbing endurance curves were calculated by the log-rank test. Data are represented as means ± SEM. *P

Figure 5. Influence of heart salt RNAi, HSI, and LTE on the climbing ability and lifespan in Drosophila. (A) The climbing index in 1-week old, 3-week old, and 5-week old flies. (B) Fly population survival (%) curve. The leftmost curve represents the Salt-control+HSI group, of which flies had the shortest lifespan. (C) Influence of heart salt RNAi and HSI on climbing endurance in 1-week old flies. (D) Influence of heart salt RNAi and HSI on climbing endurance in 3-week old flies. (E) Influence of heart salt RNAi and HSI on climbing endurance in 5-week old flies. (F) Influence of heart salt RNAi, exercise, and HSI on climbing endurance in 1-week old flies. (G) Influence of heart salt RNAi, exercise, and HSI on climbing endurance in 3-week old flies. (H) Influence of heart salt RNAi, exercise, and HSI on climbing endurance in 5-week old flies. For climbing ability measurement, the sample size was about 100 flies for each group. For climbing index, using a one-way analysis of variance (ANOVA) followed by an LSD test among different groups. For lifespan, the sample size was 200-220 flies for each group. P-values for lifespan curves and climbing endurance curves were calculated by the log-rank test. Data are represented as means ± SEM. *P<0.05; **P <0.01.