Research Paper Volume 13, Issue 17 pp 21134—21141

RNA N6-methyladenosine demethylase FTO promotes osteoporosis through demethylating Runx2 mRNA and inhibiting osteogenic differentiation

The expression level of methyltransferase FTO is elevated in BMSCs from OP patients and OVX mouse. (A) The methylated RNA (m6A) level in 20 cases of normal BMSCs from bone fracture patients (N-BMSCs) and 20 cases of BMSCs from OP patients (OP-BMSCs). *PB) The mRNA level of critical methyltransferase (METTL3, METTL14, RBM15, WTAP and VIRMA) and demethylases (FTO and ALKBH1) in 20 cases of normal BMSCs from bone fracture patients (N-BMSCs) and 20 cases of BMSCs from OP patients (OP-BMSCs) were analyzed by qRT-PCR. *PPC) Representative images of μCT reconstructive images of tibial plateau in sham and OVX groups. (D) 3D structural parameters-BMD, BV/TV, Tb.N, Tb.Sp, Tb.Th and SMI-of tibial plateau by μCT in sham and OVX groups. (E) The methylated RNA (m6A) level and FTO expression in BMSCs from sham and OVX mouse. *PF) The protein level of FTO in BMSCs from sham and OVX mouse were analyzed by western blot. **P

Figure 1. The expression level of methyltransferase FTO is elevated in BMSCs from OP patients and OVX mouse. (A) The methylated RNA (m6A) level in 20 cases of normal BMSCs from bone fracture patients (N-BMSCs) and 20 cases of BMSCs from OP patients (OP-BMSCs). *P<0.05. (B) The mRNA level of critical methyltransferase (METTL3, METTL14, RBM15, WTAP and VIRMA) and demethylases (FTO and ALKBH1) in 20 cases of normal BMSCs from bone fracture patients (N-BMSCs) and 20 cases of BMSCs from OP patients (OP-BMSCs) were analyzed by qRT-PCR. *P<0.05 and **P<0.01. (C) Representative images of μCT reconstructive images of tibial plateau in sham and OVX groups. (D) 3D structural parameters-BMD, BV/TV, Tb.N, Tb.Sp, Tb.Th and SMI-of tibial plateau by μCT in sham and OVX groups. (E) The methylated RNA (m6A) level and FTO expression in BMSCs from sham and OVX mouse. *P<0.05. (F) The protein level of FTO in BMSCs from sham and OVX mouse were analyzed by western blot. **P<0.01.