Research Paper Volume 13, Issue 16 pp 20192—20217

Hsa-circ-0007292 promotes the osteogenic differentiation of posterior longitudinal ligament cells via regulating SATB2 by sponging miR-508-3p

Hsa

Figure 8. Hsa_circ_0007292 modulates the osteogenic differentiation of OPLL cells via the miR-508-3p/SATB2 pathway. (A, B) The inhibited mRNA and protein expression of SATB2 mediated by hsa_circ_0007292 knockdown was significantly reversed by miR-508-3p inhibitor (n=3). (C) SATB2 expression was increased by the SATB2 overexpression vector (n=3). (D) The suppressed ALP staining of OPLL cells caused by miR-508-3p mimic was rescued by overexpression of SATB2. (E, F) The suppression of osteogenic differentiation at the mRNA and protein levels caused by miR-508-3p could be reversed by overexpression of SATB2(n=3). (G) The suppressed ALP staining of OPLL cells caused by si-circ-0007292 was rescued by overexpression of SATB2. (H, I) The overexpression of SATB2 attenuated the hsa_circ_0007292 knockdown-induced inhibitory effects on osteogenesis(n=3). All experiments were conducted at least three times. Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P<0.001.