Research Paper Volume 13, Issue 15 pp 19127—19144

The life in a gradient: calcium, the lncRNA SPRR2C and mir542/mir196a meet in the epidermis to regulate the aging process

ISH staining of SPRR2c and mir542-5p. (A–C) ISH staining of SPRR2C. (A) Foreskin sample from a 6-year-old; (B) Foreskin sample of a 30-year-old; (C) Foreskin sample of a 61 year old. Increased SPRR2C levels were detected in old skin samples compared to young skin. (D–F) ISH staining of mir542-5p. (D) Foreskin sample of an eight-year old; (E) Foreskin sample of a 30-year-old; (F) Foreskin sample of a 61-year old. During aging the hsa-mir542-5p levels gradually increase. (B, E) as well as (C, F) are specimen from identical donors. The intensity of the black-purple staining correlates with the amount of either SPRR2C or mir-542-5p. Stratum basale appears brownish due to the presence of melanocytes and is no specific staining. Scale bar: 50 μm.

Figure 7. ISH staining of SPRR2c and mir542-5p. (AC) ISH staining of SPRR2C. (A) Foreskin sample from a 6-year-old; (B) Foreskin sample of a 30-year-old; (C) Foreskin sample of a 61 year old. Increased SPRR2C levels were detected in old skin samples compared to young skin. (DF) ISH staining of mir542-5p. (D) Foreskin sample of an eight-year old; (E) Foreskin sample of a 30-year-old; (F) Foreskin sample of a 61-year old. During aging the hsa-mir542-5p levels gradually increase. (B, E) as well as (C, F) are specimen from identical donors. The intensity of the black-purple staining correlates with the amount of either SPRR2C or mir-542-5p. Stratum basale appears brownish due to the presence of melanocytes and is no specific staining. Scale bar: 50 μm.