Research Paper Volume 13, Issue 15 pp 19760—19775

miRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis

M2-TAM exosomes strengthened OS growth and metastasis. Il-4/13-induced M2-TAMs were co-cultured with 143B and Saos2 cells. (A) CCK-8 tested cell viability. (B) CCK-8 assayed cell proliferation. (C) Cell migration and invasion were examined by Transwell assay. (D) Cell apoptosis was determined by FCM. (E) The profiles of Bax, Bcl2 and Cleaved Caspase-3 were compared by WB. *P **P ***P N = 3.

Figure 1. M2-TAM exosomes strengthened OS growth and metastasis. Il-4/13-induced M2-TAMs were co-cultured with 143B and Saos2 cells. (A) CCK-8 tested cell viability. (B) CCK-8 assayed cell proliferation. (C) Cell migration and invasion were examined by Transwell assay. (D) Cell apoptosis was determined by FCM. (E) The profiles of Bax, Bcl2 and Cleaved Caspase-3 were compared by WB. *P < 0.05, **P < 0.01, ***P < 0.001 (vs. con group), N = 3.