Research Paper Volume 13, Issue 15 pp 19760—19775

miRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis

Overexpressing miR-221-3p aggravated OS. M2-TAMs were transfected with miR-221-3p mimics. The exosomes in the culture medium of M2-TAMs were isolated and then treated with Saos2 cells. (A) qRT-PCR detected the miR-221-3p profile. (B) CCK-8 checked cell viability. (C) Cell proliferation was tested by the colony formation assay. (D) Cell migration and invasion were examined by Transwell assay. (E) Cell apoptosis of Saos2 was detected by FCM. (F) Bax, Bcl2 and Cleaved Caspase-3 were examined by WB. *P **P ***P &P &&P &&&P TAMs+miR-NC group), N = 3.

Figure 3. Overexpressing miR-221-3p aggravated OS. M2-TAMs were transfected with miR-221-3p mimics. The exosomes in the culture medium of M2-TAMs were isolated and then treated with Saos2 cells. (A) qRT-PCR detected the miR-221-3p profile. (B) CCK-8 checked cell viability. (C) Cell proliferation was tested by the colony formation assay. (D) Cell migration and invasion were examined by Transwell assay. (E) Cell apoptosis of Saos2 was detected by FCM. (F) Bax, Bcl2 and Cleaved Caspase-3 were examined by WB. *P < 0.05, **P < 0.01, ***P < 0.001 (vs. con group). &P < 0.05, &&P < 0.01, &&&P < 0.001 (vs. EXOTAMs+miR-NC group), N = 3.