Research Paper Volume 13, Issue 15 pp 19760—19775

miRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis

miR-221-3p boosted tumor growth in tumor-bearing mice. miR-221-3p mimics were subcutaneously injected into tumor-bearing mice to observe tumor growth. (A) The tumor. (B) The tumor volume. (C) The tumor weight. (D) WB examined the SOCS3/JAK2/STAT3 pathway expression and its phosphorylated level in mouse tumor tissues. (E) IHC detected the positive numbers of Ki67, SOCS3 and Vimentin. ***P N = 5. miR-221-3p mimics were transfected into M2-TAMs. The exosomes in the culture medium of M2-TAMs were isolated and then subcutaneously injected into tumor-bearing mice to observe tumor growth. (F) The tumor tissue. (G) The tumor volume. (H) The tumor weight. (I) WB examined SOCS3/JAK2/STAT3 expression and its phosphorylated level in mouse tumor tissues. (J) IHC detected the positive numbers of Ki67, SOCS3 and Vimentin. **P ***P &&P &&&P TAMs+miR-NC group), N = 5.

Figure 8. miR-221-3p boosted tumor growth in tumor-bearing mice. miR-221-3p mimics were subcutaneously injected into tumor-bearing mice to observe tumor growth. (A) The tumor. (B) The tumor volume. (C) The tumor weight. (D) WB examined the SOCS3/JAK2/STAT3 pathway expression and its phosphorylated level in mouse tumor tissues. (E) IHC detected the positive numbers of Ki67, SOCS3 and Vimentin. ***P < 0.001 (vs. miR-NC group), N = 5. miR-221-3p mimics were transfected into M2-TAMs. The exosomes in the culture medium of M2-TAMs were isolated and then subcutaneously injected into tumor-bearing mice to observe tumor growth. (F) The tumor tissue. (G) The tumor volume. (H) The tumor weight. (I) WB examined SOCS3/JAK2/STAT3 expression and its phosphorylated level in mouse tumor tissues. (J) IHC detected the positive numbers of Ki67, SOCS3 and Vimentin. **P < 0.01, ***P < 0.001 (vs. con group); &&P < 0.01, &&&P < 0.001 (vs. EXOTAMs+miR-NC group), N = 5.