Research Paper Volume 13, Issue 15 pp 19805—19821

MiR-195-5p and miR-205-5p in extracellular vesicles isolated from diabetic foot ulcer wound fluid decrease angiogenesis by inhibiting VEGFA expression

Characteristics of EVs derived from different wound fluids. (A) TEM analysis and NTA analysis of EVs. Scale bar: 200 nm. (B) EVs-related markers were detected by Western blotting. (C) EVs were labeled with PKH26 and co-incubated with HUVECs, and the representative images photographed by confocal microscope are showed above. Scale bar: 10 μm. At least three replicates of each experiment were performed.

Figure 1. Characteristics of EVs derived from different wound fluids. (A) TEM analysis and NTA analysis of EVs. Scale bar: 200 nm. (B) EVs-related markers were detected by Western blotting. (C) EVs were labeled with PKH26 and co-incubated with HUVECs, and the representative images photographed by confocal microscope are showed above. Scale bar: 10 μm. At least three replicates of each experiment were performed.