Research Paper Volume 13, Issue 15 pp 19805—19821

MiR-195-5p and miR-205-5p in extracellular vesicles isolated from diabetic foot ulcer wound fluid decrease angiogenesis by inhibiting VEGFA expression

MiR-195-5p and miR-205-5p inhibited angiogenesis through reducing VEGFA. (A) MiR-195-5p and miR-205-5p expression level in HUVECs transfected with miRNA mimics was determined by qRT-PCR. (B, C) Transwell assay of HUVECs transfected with either or both of miR-195-5p and miR-205-5p mimics. (D, E) In vitro tube formation assay of HUVECs transfected with either or both of miR-195-5p and miR-205-5p mimics. (F, G) VEGFA-3′-UTR luciferase reporter assays of HUVECs in the presence of miR-195-5p and miR-205-5p mimics separately. (H, I) The expression level of VEGFA in HUVECs overexpressing either or both of the miRNA mimics were detected by qRT-PCR and western blot separately. (J) The concentration of VEGFA in culture medium of HUVECs overexpressing either or both of the miRNA mimics were detected by Elisa. *P **P ***P

Figure 4. MiR-195-5p and miR-205-5p inhibited angiogenesis through reducing VEGFA. (A) MiR-195-5p and miR-205-5p expression level in HUVECs transfected with miRNA mimics was determined by qRT-PCR. (B, C) Transwell assay of HUVECs transfected with either or both of miR-195-5p and miR-205-5p mimics. (D, E) In vitro tube formation assay of HUVECs transfected with either or both of miR-195-5p and miR-205-5p mimics. (F, G) VEGFA-3′-UTR luciferase reporter assays of HUVECs in the presence of miR-195-5p and miR-205-5p mimics separately. (H, I) The expression level of VEGFA in HUVECs overexpressing either or both of the miRNA mimics were detected by qRT-PCR and western blot separately. (J) The concentration of VEGFA in culture medium of HUVECs overexpressing either or both of the miRNA mimics were detected by Elisa. *P < 0.05, **P < 0.01, ***P < 0.001. At least three replicates of each experiment were performed.