Research Paper Volume 13, Issue 15 pp 19822—19834

Circular RNA ZNF609 drives tumor progression by regulating the miR-138-5p/SIRT7 axis in melanoma

CircZNF609 promotes tumor growth of melanoma cells in vivo. (A–D) The impact of circZNF609 on tumor growth of melanoma cells in vivo was analyzed by nude mice tumorigenicity assay (n = 5). The A375 cells were treated with control shRNA or circZNF609 shRNA. (A) Representative images of dissected tumors from nude mice were shown. (B) The average tumor volume was calculated and presented. (C) The average tumor weight was calculated and presented. (D) The expression of miR-138-5p was measured by qPCR in the tumor tissues of the mice. (E) The expression of SIRT7was tested by Western blot analysis in the tumor tissues of the mice. Data are presented as mean ± SD. Statistic significant differences were indicated: *P **P

Figure 7. CircZNF609 promotes tumor growth of melanoma cells in vivo. (AD) The impact of circZNF609 on tumor growth of melanoma cells in vivo was analyzed by nude mice tumorigenicity assay (n = 5). The A375 cells were treated with control shRNA or circZNF609 shRNA. (A) Representative images of dissected tumors from nude mice were shown. (B) The average tumor volume was calculated and presented. (C) The average tumor weight was calculated and presented. (D) The expression of miR-138-5p was measured by qPCR in the tumor tissues of the mice. (E) The expression of SIRT7was tested by Western blot analysis in the tumor tissues of the mice. Data are presented as mean ± SD. Statistic significant differences were indicated: *P < 0.05, **P < 0.01.