Research Paper Volume 13, Issue 15 pp 19165—19185

Endothelium-specific deletion of amyloid-β precursor protein exacerbates endothelial dysfunction induced by aging

(A) Effects of aging on protein expression of APP in the aortas of wild-type (WT) littermates and eAPP−/− mice (n=10 per group). (B) Effects of aging on ex-vivo sAPPα secretion from wild-type (WT) littermates and eAPP−/− mice aortas. The supernatants were collected and analyzed for sAPPα levels. Results were normalized against tissue protein levels (n=12 per group for young WT littermates and eAPP−/− mice and n=15 per group for aged WT littermates and eAPP−/− mice). (C) Effects of aging on protein expression of ADAM10 in the aortas of wild-type (WT) littermates and eAPP−/− mice (n=6 per group). (D) Effects of aging on protein expression of BACE1 in the aortas of wild-type (WT) littermates and eAPP−/− mice (n=6 per group). Western blot results are the relative densitometry compared with β-actin protein. All results are representing box plots with whiskers showing the median, 25th to 75th percentiles, and min-max range. * P

Figure 1. (A) Effects of aging on protein expression of APP in the aortas of wild-type (WT) littermates and eAPP−/− mice (n=10 per group). (B) Effects of aging on ex-vivo sAPPα secretion from wild-type (WT) littermates and eAPP−/− mice aortas. The supernatants were collected and analyzed for sAPPα levels. Results were normalized against tissue protein levels (n=12 per group for young WT littermates and eAPP−/− mice and n=15 per group for aged WT littermates and eAPP−/− mice). (C) Effects of aging on protein expression of ADAM10 in the aortas of wild-type (WT) littermates and eAPP−/− mice (n=6 per group). (D) Effects of aging on protein expression of BACE1 in the aortas of wild-type (WT) littermates and eAPP−/− mice (n=6 per group). Western blot results are the relative densitometry compared with β-actin protein. All results are representing box plots with whiskers showing the median, 25th to 75th percentiles, and min-max range. * P<0.05 versus young mice of same strain; † P<0.05 versus age-matched WT littermates (two-way ANOVA followed by Tukey’s HSD test).