Research Paper Volume 13, Issue 16 pp 20258—20276

DNMT3B decreases extracellular matrix degradation and alleviates intervertebral disc degeneration through TRPA1 methylation to inhibit the COX2/YAP axis

DNMT3B was expressed in IVDD tissues in low levels. (A) Histological analysis of intervertebral disc tissues of Sham rats and IVDD rats. (B) The expression of collagen II, aggrecan, MMP3, and MMP9 detected by RT-qPCR in intervertebral disc tissues of sham-operated rats and IVDD rats. (C) Western blot analysis of the expression of collagen II, aggrecan, MMP3, and MMP9 in intervertebral disc tissues of sham-operated rats and IVDD rats. (D) The levels of inflammatory factors IL-6, TNF-α, IL-8 was detected by ELISA. (E) The expression of DNMT3B in the intervertebral disc of sham-operated rats and IVDD rats was detected by RT-qPCR. (F) The expression of DNMT3B in the intervertebral disc of sham-operated rats and IVDD rats was detected by Western blot. Measurement data are expressed as the mean ± standard deviation and analyzed using independent sample t-tests. **, p

Figure 1. DNMT3B was expressed in IVDD tissues in low levels. (A) Histological analysis of intervertebral disc tissues of Sham rats and IVDD rats. (B) The expression of collagen II, aggrecan, MMP3, and MMP9 detected by RT-qPCR in intervertebral disc tissues of sham-operated rats and IVDD rats. (C) Western blot analysis of the expression of collagen II, aggrecan, MMP3, and MMP9 in intervertebral disc tissues of sham-operated rats and IVDD rats. (D) The levels of inflammatory factors IL-6, TNF-α, IL-8 was detected by ELISA. (E) The expression of DNMT3B in the intervertebral disc of sham-operated rats and IVDD rats was detected by RT-qPCR. (F) The expression of DNMT3B in the intervertebral disc of sham-operated rats and IVDD rats was detected by Western blot. Measurement data are expressed as the mean ± standard deviation and analyzed using independent sample t-tests. **, p < 0.01.