Research Paper Volume 13, Issue 16 pp 20335—20358

Proteomic characterization of secretory granules in dopaminergic neurons indicates chromogranin/secretogranin-mediated protein processing impairment in Parkinson’s disease

Verification of mRNA expressions of 56 significantly differentially expressed proteins on the secretory granules between control and MPP+ groups via real-time PCR. (A) The mRNA expressions of 38 significantly down-regulated proteins on the secretory granules were verified using real-time PCR. (B) The mRNA expressions of 18 significantly upregulated proteins on the secretory granules were verified using real-time PCR. Two-tailed unpaired Student t-tests were performed between the control and treated groups. *Statistically significant with P N = 3.

Figure 5. Verification of mRNA expressions of 56 significantly differentially expressed proteins on the secretory granules between control and MPP+ groups via real-time PCR. (A) The mRNA expressions of 38 significantly down-regulated proteins on the secretory granules were verified using real-time PCR. (B) The mRNA expressions of 18 significantly upregulated proteins on the secretory granules were verified using real-time PCR. Two-tailed unpaired Student t-tests were performed between the control and treated groups. *Statistically significant with P < 0.05; Error bars are SD; N = 3.