Research Paper Volume 13, Issue 16 pp 20372—20382

Circular RNA circRHOT1 contributes to pathogenesis of non-small cell lung cancer by epigenetically enhancing C-MYC expression through recruiting KAT5

CircRHOT1/c-MYC signaling contributes NSCLC cell proliferation in vitro and in vivo. (A and B) The A549 and H1299 cells were treated with circRHOT1 siRNA or co-treated with circRHOT1 siRNA and KAT5 overexpression vector or c-MYC overexpression vector. The cell survival was detected by Edu assays. (C–F) The nude mice (n = 5) were injected with A549 cells treated with circRHOT1 siRNA or co-treated with circRHOT1 siRNA and c-MYC overexpression vector. The tumor image (C), tumor weight (D), and tumor volume (E) were shown. (F) The Ki-67 levels were analyzed by IHC staining. mean ± SD, **P

Figure 6. CircRHOT1/c-MYC signaling contributes NSCLC cell proliferation in vitro and in vivo. (A and B) The A549 and H1299 cells were treated with circRHOT1 siRNA or co-treated with circRHOT1 siRNA and KAT5 overexpression vector or c-MYC overexpression vector. The cell survival was detected by Edu assays. (CF) The nude mice (n = 5) were injected with A549 cells treated with circRHOT1 siRNA or co-treated with circRHOT1 siRNA and c-MYC overexpression vector. The tumor image (C), tumor weight (D), and tumor volume (E) were shown. (F) The Ki-67 levels were analyzed by IHC staining. mean ± SD, **P < 0.01.