Research Paper Volume 13, Issue 15 pp 19207—19229

Hedgehog dysregulation contributes to tissue-specific inflammaging of resident macrophages

Age-related changes in the transcriptome of resident macrophages are tissue-specific. (A) The workflow with tissue-resident macrophages used to investigate physiological aging in the innate immune system. After processing and treatment, all macrophages were subjected to RNA-seq analysis and other assays as described for each experiment. (B) Clustering analysis reveals tissue-specific gene clusters dysregulated with physiological aging. An ad hoc selection of genes representing each cluster are shown on the right.

Figure 1. Age-related changes in the transcriptome of resident macrophages are tissue-specific. (A) The workflow with tissue-resident macrophages used to investigate physiological aging in the innate immune system. After processing and treatment, all macrophages were subjected to RNA-seq analysis and other assays as described for each experiment. (B) Clustering analysis reveals tissue-specific gene clusters dysregulated with physiological aging. An ad hoc selection of genes representing each cluster are shown on the right.