Figure 4. Ceruloplasmin protein levels in BRCA. (A) Ceruloplasmin protein levels in BRCA tissues and normal breast tissues were investigated based on the CPTAC database. (B–E) Relationship between ceruloplasmin protein expression and clinicopathological parameters of BRCA patients, including individual cancer stage, major subclass, patient race and age. (F) The protein levels of ceruloplasmin in MCF-10A, MDA-MB-231 and MCF-7 cells were investigated by western blotting. (G) The mRNA levels of ceruloplasmin in three different cell lines were examined by real-time PCR. (H) Representative IHC images of ceruloplasmin in normal breast tissues (upper) and BRCA (lower) were shown. The staining was quantified (normal breast tissue, n=3; BRCA, n=8). One-way ANOVA was used. *<0.05, **<0.01, ***<0.001.