Figure 3. DKK1 is identified as the target of miR-33a-5p. (A) The predicted binding sites between n DKK1 and miR-33a-5p. (B) Interaction between DKK1 and miR-33a-5p by dual luciferase reporter assay; (C) Western blot of DKK1 protein expression in TE-1 and Eca-109 cells after transfection with miR-NC, miR-33a-5p-mimics and miR-33a-5p-inhibitor and the protein diagram. The inter-group comparison was conducted by the independent sample t-test, mean ± SD, bP < 0.01, cP <0.05, and dP < 0.01 for inter-group comparisons.