Research Paper Volume 13, Issue 16 pp 20481—20494

miR-33a-5p inhibits the progression of esophageal cancer through the DKK1-mediated Wnt/β-catenin pathway

Effects of DKK1 on biological function of esophageal cancer cells. (A) qRT-PCR detection of DKK1 expression in TE-1 and Eca-109 after transfection of si-NC, si-DKK1, and sh-DKK1; (B) CCK-8 assay of cell proliferation of TE-1 and Eca-109; (C) Migration number of E-1 and Eca-109 cells by Transwell assay; (D) Invasion number of TE-1 and Eca-109 cells by Transwell assay; (E) Cells apoptosis rates of TE-1 and Eca-109 cells by Flow cytometry and the apoptosis diagram; (F) Western blot of Cyclin D1, c-jun, CD44 and c-Met protein expression in TE-1 and Eca-109 cells and the protein bands. The inter-group comparison was conducted by the independent sample t-test, multiple time point data were analyzed by repeated measures analysis of variance (ANOVA), mean ± SD, bP cP dP

Figure 4. Effects of DKK1 on biological function of esophageal cancer cells. (A) qRT-PCR detection of DKK1 expression in TE-1 and Eca-109 after transfection of si-NC, si-DKK1, and sh-DKK1; (B) CCK-8 assay of cell proliferation of TE-1 and Eca-109; (C) Migration number of E-1 and Eca-109 cells by Transwell assay; (D) Invasion number of TE-1 and Eca-109 cells by Transwell assay; (E) Cells apoptosis rates of TE-1 and Eca-109 cells by Flow cytometry and the apoptosis diagram; (F) Western blot of Cyclin D1, c-jun, CD44 and c-Met protein expression in TE-1 and Eca-109 cells and the protein bands. The inter-group comparison was conducted by the independent sample t-test, multiple time point data were analyzed by repeated measures analysis of variance (ANOVA), mean ± SD, bP < 0.01, cP < 0.05, and dP < 0.01 for inter-group comparisons.