Figure 1. (A) Positive apoptotic neural cell was identified with 4′,6-diamidino-2-phenylindole (DAPI) staining and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The upper image with blue spots indicates DAPI staining, middle image with green spots indicates TUNEL assay, and lower image with blue and green spots indicates Merge of DAPI staining and TUNEL assay. Scale bar, 50μm. (B) The bar shows the quantification of TUNEL apoptosis (%), determined as the proportion of TUNEL-positive cells relative to total DAPI stained nuclei. **: p<0.01 in comparison between SHR group and WKY rat group. #: p<0.05; in comparison between SHR-EX group and SHR group.