Figure 6. The IGF-1-related survival pathway in a normotensive Wistar Kyoto (WKY) group, a spontaneously early aged hypertensive (SHR) group and a hypertension with 12 weeks exercise training (SHR-EX) group. (A) The representative protein levels of IGF-1, PI3K, p-PI3K, AKT and p-AKT protein extracted from the cerebral cortex of excised brain in WKY, SHR, and SHR-EX groups were measured by western blot analysis. The α-tubulin was used as an internal control. (B) Bars represent the relative changes of protein quantification in IGF-1, p-PI3K/PI3K, and pAKT/AKT on α-tubulin and mean values ± SD (n=6 in each group). **: p<0.01, ***: p<0.001, in comparison between SHR group and WKY rat group; ##: p<0.01, ###: p<0.001, in comparison between SHR-EX group and SHR group.